NoE members key lab techniques
Göran Akusjärvi |
| goran.akusjarvi@imbim.uu.se |
| Key Lab Techniques |
| Nuclear extract preparation, in vitro splicing, virus infection, transient transfection, in vivo reporter gene analysis (CAT, Luc, S1, RT-PCR, Northern, Western), construction of recombinant adenovirus vectors, ChIP analysis | Members Index |
Rolf Apweiler |
| apweiler@ebi.ac.uk |
| Key Lab Techniques |
| Not available | Members Index |
Gil Ast |
| gilast@post.tau.ac.il |
| Key Lab Techniques |
| Comparative genomics, in vivo minigene analysis, datasets of homologous exons and introns | Members Index |
Didier Auboeuf |
| auboeuf@stlouis.inserm.fr |
| Key Lab Techniques |
| Dependant cancers cell lines, expression of vectors of nuclear receptor and transcriptional coregulators, hormone-dependant reporter genes | Members Index |
Andrea Barta |
| Andrea.Barta@medunivie.ac.at |
| Key Lab Techniques |
| protein-protein interactions, RNA structure analysis, confocal microscopy, Arabidopsis transformation, plant tissue culture, gene expression analysis in plants, in vitro splicing assays | Members Index |
Francisco E. Baralle |
| baralle@icgeb.org |
| Key Lab Techniques |
| siRNAs, anti hnRNP, SR antibodies, expression of splicing factors in mammalian and eukaryotic systems | Members Index |
Jean Beggs |
| j.beggs@ed.ac.uk |
| Key Lab Techniques |
| yeast in vitro splicing assays, qPCR, yeast intron micro-array, two-hybrid screening, protein:protein interactions, fluorescence microscopy with yeast, Retinitis pigementosa | Members Index |
Peer Bork |
| bork@embl.de |
| Key Lab Techniques |
| Computational biology tools | Members Index |
Giuseppe Biamonti |
| biamonti@igm.cnr.it |
| Key Lab Techniques |
| Stable cell lines expressing T7-SF2/ASF and mutants, expression cDNAs, SAF-B antisera, SatIII RNA | Members Index |
Albrecht Bindereif |
| albrecht.bindereif@chemie.bio.uni-giessen.de |
| Key Lab Techniques |
| In vivo minigene analysis, in vitro splicing, affinity purification of RNPs | Members Index |
Christiane Branlant |
| Christiane.Branlant@maem.uhp-nancy.fr |
| Key Lab Techniques |
| RNP purification using tagged RNAs, RNA and RNP structure analysis in solution, by X-ray cristallography and by in silico modelisation, in vitro and in cellulo experiments for splicing analysis | Members Index |
John W. S. Brown |
| john.brown@scri.ac.uk |
| Key Lab Techniques |
| In vivo systems for splicing analysis; medium through-put RT-PCR | Members Index |
Javier F. Caceres |
| Javier.Caceres@hgu.mrc.ac.uk |
| Key Lab Techniques |
| In vitro splicing, in vivo minigene analysis, two-hybrid screening, reporter gene construction, CLIP (UV cross-linking and immunoprecipitation), RNA interference in C.elegans | Members Index |
Maria Carmo-Fonseca |
| carmo.fonseca@fm.ul.pt |
| Key Lab Techniques |
| Confocal fluorescence microscopy, FISH, immunofluorescence and GFP-fusion protein analysis, FRAP, FRET, RNAi | Members Index |
Ian Eperon |
| eci@le.ac.uk |
| Key Lab Techniques |
| Targeted oligonucleotide enhancers of splicing (TOES); time-resolved psoralen cross-linking; quantitative RNase H assays to measure dissociation rates of U1 snRNPs etc.; use of RNase H to measure simultaneous occupancy of multiple sites | Members Index |
Davide Gabellini |
| gabellini.davide@hsr.it |
| Key Lab Techniques |
| Mouse transgenics, nuclear extract preparation and fractionation, virus infection, transient and stable transfection, in vivo reporter gene analysis, ChrIP analysis, one and two hybrid screening, protein:protein interaction, RNAi by short hairpin (sh)RNAs | Members Index |
Artur Jarmolowski |
| artjarmo@amu.edu.pl |
| Key Lab Techniques |
| plant transformation, RNAi technology, yeast two hybrid system, splicing assays in transfected tobacco protoplasts, recombinant protein expression and purification, subcellular localization of proteins, protein/protein and protein/RNA interactions, RNA modification | Members Index |
Jørgen Kjems |
| jk@mb.au.dk |
| Key Lab Techniques |
| In vitro and in vivo RNA processing assays, chemical and enzymatic modification of RNA, single molecule fluorescence microscopy, live cell imaging, RNAi, oligonucleotide delivery in vitro and in vivo (mouse models), flip-in system, small angle X-ray scattering, atomic force microscopy, protein expression | Members Index |
Alberto R. Kornblihtt |
| ark@fbmc.fcen.uba.ar |
| Key Lab Techniques |
| In vivo minigene analysis, transient transfections of mammalian cells, real time PCR, immunofluorescence, Chromatin Immunoprecipitation (ChIP) | Members Index |
Angela Krämer |
| angela.kraemer@cellbio.unige.ch |
| Key Lab Techniques |
| In vitro and in vivo splicing, protein-protein interactions, protein-RNA interactions, RNA interference, CLIP technology | Members Index |
Angus I. Lamond |
| angus@lifesci.dundee.ac.uk |
| Key Lab Techniques |
| Fluoresence imaging, FRET, FLIM, quantitative mass spectrometry, nuclear fractionation and pre-mRNA splicing assays | Members Index |
Reinhard Lührmann |
| reinhard.luehrmann@mpi-bpc.mpg.de |
| Key Lab Techniques |
| Antibodies, biochemical purification, EM, eukaryotic cell culture, cutting edge microscopy, mass-spectrometry, recombinant protein expression, RNA structure probing, RNA synthesis, RNAi, x-ray structural analysis, yeast genetics | Members Index |
Karla M. Neugebauer |
| neugebau@mpi-cbg.de |
| Key Lab Techniques |
| Chromatin immunoprecipitation (ChIP), RNA immunoprecipitation (RIP), fluorescent tagging of splicing factors (GFP, CFP, YFP, photo-activatable GFP, others) for live imaging, FRAP, FRET, FCS etc. Tagging of splicing factors on bacterial artificial chromosomes (BACs) for transient and stable expression in mammalian cells. RNAi (especially esiRNA, retrovirus mediated RNAi) | Members Index |
Daniel Schümperli |
| daniel.schuemperli@izb.unibe.ch |
| Key Lab Techniques |
| In vivo minigene analysis, two hybrid screening, reporter gene construction, protein:protein interaction, protein:RNA interaction, lentiviral vectors, RNAi by short hairpin (sh)RNAs, -thalassemia, spinal muscular atrophy, HIV/AIDS | Members Index |
Bertrand Seraphin |
| seraphin@cgm.cnrs-gif.fr |
| Key Lab Techniques |
| Commitment complexes, reporter gene, protein:protein interaction, protein-RNA interaction, protein complexes, RNA quality control, NMD, nuclear RNA decay, yeast Saccharomyces cerevisiae, mammalian cells, protein interaction methods, in vitro systems, recombinant factors | Members Index |
Chris Smith |
| cwjs1@cam.ac.uk |
| Key Lab Techniques |
| Minigene construction, transfection and analysis in cultured cells. RNA interference, 2D-Difference Gel Electrophoresis (Cambridge Centre for Proteomics) | Members Index |
Hermona Soreq |
| soreq@cc.huji.ac.il |
| Key Lab Techniques |
| Transgenic engineering, Antisense technology, cDNA microarray | Members Index |
Stefan Stamm |
| Stefan@stamms-lab.net |
| Key Lab Techniques |
| In vivo minigene analysis, two hybrid screening, reporter gene construction, protein:protein interaction, gateway system, Alzheimer Disease, spinal muscular atrophy, Prader-Willi-Syndrome | Members Index |
James Stévenin |
| stevenin@igbmc.u-strasbg.fr |
| Key Lab Techniques |
| In vitro splicing assays, protein purification (baculovirus production), production of monoclonal and polyclonal antibodies, immunoprecipitation, SELEX, RNA-protein interactions, RNA chromatography and mass spectrometry, in vivo minigene analysis (transient transfection) | Members Index |
Jamal Tazi |
| jamal.tazi@igmm.cnrs.fr |
| Key Lab Techniques |
| Biochemistry: RNA-protein interactions, in vitro splicing assays, protein purification, RNA purification and analysis | Members Index |
Glauco P. Tocchini-Valentini |
| gtocchini@ibc.cnr.it |
| Key Lab Techniques |
| tRNA endonucleases; Mouse mutagenesis; Cre-lox technology; Archaeaexpress; GPR 37 expression; Parkinson Disease | Members Index |
Henning Urlaub |
| henning.urlaub@mpi-bpc.mpg.de |
| Key Lab Techniques |
| protein-RNA cross-linking, chromatography, MALDI/ESI mass spectrometry, proteomics | Members Index |
Juan Valcárcel |
| juan.valcarcel@crg.es |
| Key Lab Techniques |
| In vivo minigene analyses, RNA-protein interactions, in vitro splicing assays, microarray analyses | Members Index |
Mihaela Zavolan |
| mihaela.zavolan@unibas.ch |
| Key Lab Techniques |
| Algorithms for spliced alignment, functional annotation, RNA secondary structure prediction, gene prediction, discovery of regulatory motifs | Members Index |
