New paper from Karla Neugebauer's lab

Congratulations to Karla Neugebauer and her group on the publication of their new paper in the Molecular Cell:

SR protein family members display diverse activities in the formation of nascent and mature mRNPs in vivo.
Sapra AK, Ankö ML, Grishina I, Lorenz M, Pabis M, Poser I, Rollins J, Weiland EM, Neugebauer KM.
Mol Cell. 2009 Apr 24;34(2):179-90.

Abstract: The SR proteins are a family of pre-mRNA splicing factors with additional roles in gene regulation. To investigate individual family members in vivo, we generated a comprehensive panel of stable cell lines expressing GFP-tagged SR proteins under endogenous promoter control. Recruitment of SR proteins to nascent FOS RNA was transcription dependent and RNase sensitive, with unique patterns of accumulation along the gene specified by the RNA recognition motifs (RRMs). In addition, all SR protein interactions with Pol II were RNA dependent, indicating that SR proteins are not preassembled with Pol II. SR protein interactions with RNA were confirmed in situ byFRET/FLIM. Interestingly,SC35-GFPalsoexhibited FRET with DNA and failed to associate with cytoplasmicmRNAs, whereas all other SR proteins underwentnucleocytoplasmic shuttlingandassociatedwith specific nuclear and cytoplasmic mRNAs. Because different constellations of SR proteins bound nascent, nuclear, and cytoplasmic mRNAs, mRNP remodeling must occur throughout an mRNA’s lifetime.