Research Focus
We are interested in mammalian mRNA splicing, in particular in the regulation of alternative splicing. We have uncovered the role of CA-repeat and CA-rich sequences as novel intronic regulatory elements. These RNA elements are very widespread in the human genome, and we have demonstrated for several human genes, that CA RNA elements can function either as splicing enhancers or silencers, regulating the efficiency of the reaction or the splice site choice (alternative splicing). Heterogeneous nuclear RNA-binding protein (hnRNP) L binds to these elements and likely acts as a global regulator of alternative splicing.
Publications
- Palfi, Z., Jaé, N., Preusser, C., Kaminska, K.H., Bujnicki, J.M., Lee, J.H., Günzl, A., Kambach, C., Urlaub, H., Bindereif, A. (2009). SMN-assisted assembly of snRNP-specific Sm cores in trypanosomes. Genes and Development 23(14), 1650-64.
- Rajan, P., Dalgliesh, C., Bourgeois, C.F., Heiner, M., Emami, K., Clark, E.L., Bindereif, A., Stévenin, J., Robson, C.N., Leung, H.Y., Elliott, D.J. (2009). Proteomic identification of heterogeneous nuclear ribonucleoprotein L as a novel component of SLM/Sam68 Nuclear Bodies. BMC Cell Biology 10, 82.
- Rossbach, O., Hung, L.H., Schreiner, S., Grishina, I., Heiner, M., Hui, J., Bindereif, A. (2009) Auto- and crossregulation of the hnRNP L proteins by alternative splicing. Molecular and Cellular Biology 29(6), 1442-51. [epub ahead of print]
- Hui, J., Hung, L.-H., Heiner, M., Schreiner, S., Neumüller, N., Reither, G., Haas, S.A., and Bindereif, A. (2005). Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO Journal 24, 1988-1998.
- Medenbach, J., Schreiner, S., Liu, S., Lührmann, R., and Bindereif, A. (2004). Human U4/U6 snRNP recycling factor p110: mutational analysis reveals function of TPR domain in recycling. Molecular and Cellular Biology 24, 7392-7401.
- Damianov, A., Schreiner, S., and Bindereif, A. (2004). Recycling of the U12-type spliceosome requires p110, a component of the U6atac snRNP. Molecular and Cellular Biology 24, 1700-1708.
- Hui, J., Stangl, K., Lane, W.S., and Bindereif, A. (2003). HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats. Nature Structural & Molecular Biology 10, 33-37.
- Bell, M., Schreiner, S., Damianov, A., Reddy, R., and Bindereif, A. (2002). p110, a novel human U6 snRNP protein and U4/U6 snRNP recycling factor. EMBO Journal 21, 2724-2735.
Key lab techniques: in vivo minigene analysis, in vitro splicing, affinity purification of RNPs.
Key lab reagents: minigene and splicing constructs.
Lab contact: Albrecht Bindereif: albrecht.bindereif@chemie.bio.uni-giessen.de
Lab website: www.uni-giessen.de/~gf1213/index.html
|
