NoE members key lab techniques

A-B
Akusjärvi, Göran
Apweiler, Rolf
Ast, Gil
Auboeuf, Didier

Barta, Andrea
Baralle, Francisco E.
Beggs, Jean
Bork, Peer
Biamonti, Giuseppe
Bindereif, Albrecht
Branlant, Christiane
Brown, John W. S.

C-K
Caceres, Javier F.
Carmo-Fonseca, Maria

Eperon, Ian

Gabellini, Davide

Jarmolowski, Artur

Kjems, Jørgen
Kornblihtt, Alberto

Krämer, Angela R.

L-S
Lamond, Angus I.
Lührmann, Reinhard

Neugebauer, Karla

Schümperli, Daniel M.
Seraphin, Bertrand
Smith, Chris
Soreq, Hermona
Stamm, Stefan
Stévenin, James

T-Z
Tazi, Jamal
Tocchini-Valentini, Glauco P.

Urlaub, Henning

Valcárcel, Juan

Zavolan, Mihaela

Göran Akusjärvi

goran.akusjarvi@imbim.uu.se
Key Lab Techniques
Nuclear extract preparation, in vitro splicing, virus infection, transient transfection, in vivo reporter gene analysis (CAT, Luc, S1, RT-PCR, Northern, Western), construction of recombinant adenovirus vectors, ChIP analysis
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Rolf Apweiler

apweiler@ebi.ac.uk
Key Lab Techniques
Not available
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Gil Ast

gilast@post.tau.ac.il
Key Lab Techniques
Comparative genomics, in vivo minigene analysis, datasets of homologous exons and introns
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Didier Auboeuf

auboeuf@stlouis.inserm.fr
Key Lab Techniques
Dependant cancers cell lines, expression of vectors of nuclear receptor and transcriptional coregulators, hormone-dependant reporter genes
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Andrea Barta

Andrea.Barta@medunivie.ac.at
Key Lab Techniques
protein-protein interactions, RNA structure analysis, confocal microscopy, Arabidopsis transformation, plant tissue culture, gene expression analysis in plants, in vitro splicing assays
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Francisco E. Baralle

baralle@icgeb.org
Key Lab Techniques
siRNAs, anti hnRNP, SR antibodies, expression of splicing factors in mammalian and eukaryotic systems
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Jean Beggs

j.beggs@ed.ac.uk
Key Lab Techniques
yeast in vitro splicing assays, qPCR, yeast intron micro-array, two-hybrid screening, protein:protein interactions, fluorescence microscopy with yeast, Retinitis pigementosa
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Peer Bork

bork@embl.de
Key Lab Techniques
Computational biology tools
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Giuseppe Biamonti

biamonti@igm.cnr.it
Key Lab Techniques
Stable cell lines expressing T7-SF2/ASF and mutants, expression cDNAs, SAF-B antisera, SatIII RNA
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Albrecht Bindereif

albrecht.bindereif@chemie.bio.uni-giessen.de
Key Lab Techniques
In vivo minigene analysis, in vitro splicing, affinity purification of RNPs
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Christiane Branlant

Christiane.Branlant@maem.uhp-nancy.fr
Key Lab Techniques
RNP purification using tagged RNAs, RNA and RNP structure analysis in solution, by X-ray cristallography and by in silico modelisation, in vitro and in cellulo experiments for splicing analysis
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John W. S. Brown

john.brown@scri.ac.uk
Key Lab Techniques
In vivo systems for splicing analysis; medium through-put RT-PCR
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Javier F. Caceres

Javier.Caceres@hgu.mrc.ac.uk
Key Lab Techniques
In vitro splicing, in vivo minigene analysis, two-hybrid screening, reporter gene construction, CLIP (UV cross-linking and immunoprecipitation), RNA interference in C.elegans
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Maria Carmo-Fonseca

carmo.fonseca@fm.ul.pt
Key Lab Techniques
Confocal fluorescence microscopy, FISH, immunofluorescence and GFP-fusion protein analysis, FRAP, FRET, RNAi
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Ian Eperon

eci@le.ac.uk
Key Lab Techniques
Targeted oligonucleotide enhancers of splicing (TOES); time-resolved psoralen cross-linking; quantitative RNase H assays to measure dissociation rates of U1 snRNPs etc.; use of RNase H to measure simultaneous occupancy of multiple sites
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Davide Gabellini

gabellini.davide@hsr.it
Key Lab Techniques
Mouse transgenics, nuclear extract preparation and fractionation, virus infection, transient and stable transfection, in vivo reporter gene analysis, ChrIP analysis, one and two hybrid screening, protein:protein interaction, RNAi by short hairpin (sh)RNAs
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Artur Jarmolowski

artjarmo@amu.edu.pl
Key Lab Techniques
plant transformation, RNAi technology, yeast two hybrid system, splicing assays in transfected tobacco protoplasts, recombinant protein expression and purification, subcellular localization of proteins, protein/protein and protein/RNA interactions, RNA modification
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Jørgen Kjems

jk@mb.au.dk
Key Lab Techniques
In vitro and in vivo RNA processing assays, chemical and enzymatic modification of RNA, single molecule fluorescence microscopy, live cell imaging, RNAi, oligonucleotide delivery in vitro and in vivo (mouse models), flip-in system, small angle X-ray scattering, atomic force microscopy, protein expression
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Alberto R. Kornblihtt

ark@fbmc.fcen.uba.ar
Key Lab Techniques
In vivo minigene analysis, transient transfections of mammalian cells, real time PCR, immunofluorescence, Chromatin Immunoprecipitation (ChIP)
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Angela Krämer

angela.kraemer@cellbio.unige.ch
Key Lab Techniques
In vitro and in vivo splicing, protein-protein interactions, protein-RNA interactions, RNA interference, CLIP technology
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Angus I. Lamond

angus@lifesci.dundee.ac.uk
Key Lab Techniques
Fluoresence imaging, FRET, FLIM, quantitative mass spectrometry, nuclear fractionation and pre-mRNA splicing assays
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Reinhard Lührmann

reinhard.luehrmann@mpi-bpc.mpg.de
Key Lab Techniques
Antibodies, biochemical purification, EM, eukaryotic cell culture, cutting edge microscopy, mass-spectrometry, recombinant protein expression, RNA structure probing, RNA synthesis, RNAi, x-ray structural analysis, yeast genetics
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Karla M. Neugebauer

neugebau@mpi-cbg.de
Key Lab Techniques
Chromatin immunoprecipitation (ChIP), RNA immunoprecipitation (RIP), fluorescent tagging of splicing factors (GFP, CFP, YFP, photo-activatable GFP, others) for live imaging, FRAP, FRET, FCS etc. Tagging of splicing factors on bacterial artificial chromosomes (BACs) for transient and stable expression in mammalian cells. RNAi (especially esiRNA, retrovirus mediated RNAi)
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Daniel Schümperli

daniel.schuemperli@izb.unibe.ch
Key Lab Techniques
In vivo minigene analysis, two hybrid screening, reporter gene construction, protein:protein interaction, protein:RNA interaction, lentiviral vectors, RNAi by short hairpin (sh)RNAs, -thalassemia, spinal muscular atrophy, HIV/AIDS
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Bertrand Seraphin

seraphin@cgm.cnrs-gif.fr
Key Lab Techniques
Commitment complexes, reporter gene, protein:protein interaction, protein-RNA interaction, protein complexes, RNA quality control, NMD, nuclear RNA decay, yeast Saccharomyces cerevisiae, mammalian cells, protein interaction methods, in vitro systems, recombinant factors
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Chris Smith

cwjs1@cam.ac.uk
Key Lab Techniques
Minigene construction, transfection and analysis in cultured cells. RNA interference, 2D-Difference Gel Electrophoresis (Cambridge Centre for Proteomics)
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Hermona Soreq

soreq@cc.huji.ac.il
Key Lab Techniques
Transgenic engineering, Antisense technology, cDNA microarray
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Stefan Stamm

Stefan@stamms-lab.net
Key Lab Techniques
In vivo minigene analysis, two hybrid screening, reporter gene construction, protein:protein interaction, gateway system, Alzheimer Disease, spinal muscular atrophy, Prader-Willi-Syndrome
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James Stévenin

stevenin@igbmc.u-strasbg.fr
Key Lab Techniques
In vitro splicing assays, protein purification (baculovirus production), production of monoclonal and polyclonal antibodies, immunoprecipitation, SELEX, RNA-protein interactions, RNA chromatography and mass spectrometry, in vivo minigene analysis (transient transfection)
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Jamal Tazi

jamal.tazi@igmm.cnrs.fr
Key Lab Techniques
Biochemistry: RNA-protein interactions, in vitro splicing assays, protein purification, RNA purification and analysis
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Glauco P. Tocchini-Valentini

gtocchini@ibc.cnr.it
Key Lab Techniques
tRNA endonucleases; Mouse mutagenesis; Cre-lox technology; Archaeaexpress; GPR 37 expression; Parkinson Disease
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Henning Urlaub

henning.urlaub@mpi-bpc.mpg.de
Key Lab Techniques
protein-RNA cross-linking, chromatography, MALDI/ESI mass spectrometry, proteomics
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Juan Valcárcel

juan.valcarcel@crg.es
Key Lab Techniques
In vivo minigene analyses, RNA-protein interactions, in vitro splicing assays, microarray analyses
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Mihaela Zavolan

mihaela.zavolan@unibas.ch
Key Lab Techniques
Algorithms for spliced alignment, functional annotation, RNA secondary structure prediction, gene prediction, discovery of regulatory motifs
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